Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
7140804 | Sensors and Actuators B: Chemical | 2018 | 7 Pages |
Abstract
We present a novel approach for the synthesis of unique template free protein stabilized gold nanocubes (PSGNCs) for sensing and quantification of alkaline phosphatase (ALP) in biological fluids. The unique wavelength independent excitation of PSGNCs and absorption of the final product of ALP catalysis, i.e., p-Nitrophenol (p-NP) at 405â¯nm was exploited for the inner filter effect (IFE) as a sensitive and efficient method to detect ALP. The limit of detection in aqueous solution and serum was found to be 1.616â¯U/L and 9.441â¯U/L, respectively.
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Amit Kumar Sharma, Sunil Pandey, M. Shahnawaz Khan, Hui-Fen Wu,