Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
7141021 | Sensors and Actuators B: Chemical | 2018 | 23 Pages |
Abstract
Monitoring the level of cholesterol in blood serum is a critical approach for diseases surveillance. In this work, we reported on the successful construction of a new electrochemiluminescence (ECL) sensor for cholesterol detection by employing the Ag-(bovine serum albumin) BSA-MnO2 nanosheets for cholesterol oxidase (ChOx) immobilization and catalytic amplification of ECL signal. The AgNPs-BSA-MnO2 nanosheets were synthesized by using BSA as protective agent, which greatly facilitated the immobilization of ChOx due to the existing amino group donated by BSA. The structure and properties of the AgNPs-BSA-MnO2 nanosheets were characterized by a series of techniques, including transmission electron microscope (TEM), scanning electron microscope (SEM), energy-dispersive X-ray spectroscopy (EDS), powder X-ray diffraction (XRD) and Fourier-transform infrared spectroscopy (FT-IR). Importantly, experiment results indicated that the synergistic effect of AgNPs with MnO2 nanosheets could provide excellent catalytic ability towards ROSs generation and thus to efficiently amplify the ECL signal of luminol-H2O2 system. Under optimal conditions, the ECL intensity increased with the increasing cholesterol concentration in a dynamic range from 0.21â¯Î¼M to 1667â¯Î¼M with a limit of detection (LOD) of 0.07â¯Î¼M (3Ï).
Related Topics
Physical Sciences and Engineering
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Analytical Chemistry
Authors
Da-Peng Yang, Weiwei Guo, Zhengfa Cai, Yisong Chen, Xingsheng He, Chusen Huang, Junyang Zhuang, Nengqin Jia,