Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
7141969 | Sensors and Actuators B: Chemical | 2018 | 33 Pages |
Abstract
Development of fluorescent glycopolymeric probes with high water solubility, multivalency, stimuli responsiveness, high cellular internalization efficiency and good photostability, is in urgent demand. Aggregation Induced Emission (AIE) property of glucose based acrylamides (Glc-acryl and Glc-bis) have been studied at various pH, concentration and their application towards lectin recognition, bacterial detection and cell imaging have been probed. The emissions of glycoacrylamides are found to be pH dependent and exhibit Aggregation Induced Emission Enhancement (AIEE) in t-butanol-water mixtures. Fluorescent assay unravelled the specific recognition property of pendant glucose residues on glycoacrylamides towards protein, Concanavalin A (Con A), with an association constant (Ka) of 144Â ÃÂ 103Â Mâ1 and 76.3Â ÃÂ 103Â Mâ1, for the interaction with Glc-acryl and Glc-bis, respectively. The limit of detection (LOD) of Con A by Glc-acryl and Glc-bis was found to be 7.85 pM and 3.49Â nM, respectively. Incubation with E.coli revealed that the relationship between the fluorescence signal and E.coli concentration was linear from (1.00Â ÃÂ 106) to (1.70Â ÃÂ 108) cells/mL (R2Â =Â 0.98) with LOD (at S/N ratio of 3) of E.coli being 7.30Â ÃÂ 105 cells/mL. The cell penetration studies of synthesized glycoacrylamides with INT407 cell lines showed good cellular uptake.
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Juby K. Ajish, K.S. Ajish Kumar, Aakanksha Ruhela, Mahesh Subramanian, Anand Damodar Ballal, Manmohan Kumar,