Smartphone-based chemiluminescence sensing for TLC imaging

Here in, we reported the use of smartphones for quantitative chemiluminescence detection of morphine as a model analyte on a TLC plate. A device was fabricated in the laboratory using low-cost materials in a simple by-hand way. To perform chemiluminescence reaction, potassium permanganate reagent was added on the analyte spot already applied on the TLC plate, and the emitted light was captured by a smartphone camera. The key experimental parameters affecting the sensitivity of the method were investigated and optimized. The detection limit and the linear dynamic range of the method were 0.5 mg L−1 and 1.0-25 mg L−1, respectively. Relative standard deviations were 2.1 and 4.8% for the concentrations of 5.0 and 20 mg L−1, respectively. The method was simple and cost-effective, with adequate analytical performance for rapid detection of morphine.