Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
7143243 | Sensors and Actuators B: Chemical | 2016 | 8 Pages |
Abstract
A simple, rapid and sensitive method was developed using silver nanoparticles (Ag-NPs) amplified flow-based peroxyoxalate chemiluminescence system to detect berberine, an anticancer agent. Berberine could dramatically sensitize the emission from oxidation reaction of bis (2,4,6-tricholorophenyl) oxalate (TCPO) with H2O2 catalyzed by silver nanoparticles(Ag-NPs). Under optimized experimental conditions, CL intensity was found to be proportional to the concentration of berberine in the range from 5 Ã 10â9 to 5 Ã 10â7 mol Lâ1 (r > 0.9843, n = 8) was obtained with a detection limit of 8.7 Ã 10â10 mol Lâ1. Potential interference by common excipients was also studied. The relative standard deviation for 8 repetitive determinations was less than 3.4% and recoveries of 97.7% and 101.6% were obtained. The Ag nanoparticles were synthesized using Oxalis corniculata plant as green route and characterized using field emission scanning electron microscopy, particle size analyzer and UV-vis Spectroscopy. The method was successfully applied to detect berberine in pharmaceutical formulations.
Keywords
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Pourya Biparva, Seyed Mohammad Abedirad, Sayed Yahya Kazemi, Maryam Shanehsaz,