Article ID Journal Published Year Pages File Type
7143365 Sensors and Actuators B: Chemical 2016 5 Pages PDF
Abstract
We report a colorimetric detection of functional botulinum neurotoxin (BoNT) based on its proteolytic activity. Active BoNT can enzymatically cleave SNAptide, a short peptide with 15 amino acids. The cleaved peptides subsequently induced the agglomeration of gold nanoparticles, allowing a colorimetric signal that can be discerned by the naked eye at the low concentration of 1 ng/mL (6.67 pM), and achieve low limit of detection at 0.25 ng/mL (1.67 pM 3 σ/s) by spectral absorbance. This colorimetric detection will provide a simple and promising method for applications at low-resource sites.
Related Topics
Physical Sciences and Engineering Chemistry Analytical Chemistry
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