Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
7143365 | Sensors and Actuators B: Chemical | 2016 | 5 Pages |
Abstract
We report a colorimetric detection of functional botulinum neurotoxin (BoNT) based on its proteolytic activity. Active BoNT can enzymatically cleave SNAptide, a short peptide with 15 amino acids. The cleaved peptides subsequently induced the agglomeration of gold nanoparticles, allowing a colorimetric signal that can be discerned by the naked eye at the low concentration of 1Â ng/mL (6.67Â pM), and achieve low limit of detection at 0.25Â ng/mL (1.67Â pM 3 Ï/s) by spectral absorbance. This colorimetric detection will provide a simple and promising method for applications at low-resource sites.
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Authors
Shan Chen, Lok Ting Chu, Ting-Hsuan Chen,