Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
7143744 | Sensors and Actuators B: Chemical | 2016 | 9 Pages |
Abstract
We present a high-yield electro-migration method to isolate extracellular vesicles (EVs), which achieves ultracentrifugation (U/C)-level size exclusion from biological fluids (e.g., plasma). An electric field applied across a dialysis membrane with an appropriate pore size (30Â nm), facilitates protein migration through the membrane, but captures EVs on the membrane surface. Compared to conventional procedures, this method shows up to 65% EV recovery estimated at the RNA level (â¼7.9 times better than U/C) and up to 83.6% protein removal (residual protein amount is approximately half of the precipitate) in â¼30Â min (â¼9 times faster than U/C). With optimized working range of voltage and use of biochemically stable buffers, isolated EV are fully compatible with biological post-processes and assays.
Keywords
DLSExosomeGFPTBSNTAPVDFRT-PCRIPADMEMPBSBSAbovine serum albuminIsopropanolNanoparticle tracking analysisTris-buffered salineEnzyme-linked immunosorbent assayELISATAESeparationNanoporous membranePhosphate buffered salineUltracentrifugationBody fluidDulbecco’s modified eagle’s mediumreverse transcription polymerase chain reactionextracellular vesiclePurificationDynamic Light ScatteringGreen fluorescence proteinpolyethylene glycolPolyvinylidene fluoridePEG
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Siwoo Cho, Wonju Jo, Youhee Heo, Ji Yoon Kang, Rhokyun Kwak, Jaesung Park,