Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
7143754 | Sensors and Actuators B: Chemical | 2016 | 26 Pages |
Abstract
We reported a novel ECL immunosensor for detection of carcinoembryonic antigen (CEA) based on the reduced graphene Oxide-BaYF5:Yb, Er (rGO-BaYF5:Yb, Er) nanocomposites. The rGO-BaYF5:Yb, Er nanocomposites, used as electrode luminescence materials, were prepared by in situ hydrothermal method, and characterized by TEM, XRD, UV-vis spectra, and upconversion fluorescence spectra. Further, poly (diallyldimethylammonium chloride) (PDDA), Au nanoparticles (AuNPs), and Anti-CEA were successively assembled on the surface of rGO-BaYF5:Yb, Er nanocomposites modified-electrode for fabricating ECL immunosensor. The results of the ECL measurements of CEA indicated that this novel ECL immunosensor exhibited high sensitive response to CEA in a linear range of 0.001-80 ng mLâ1 with a low detection limit of 0.87 pg mLâ1, good selectivity and satisfactory stability.
Related Topics
Physical Sciences and Engineering
Chemistry
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Authors
Linlin Zhao, Juan Li, Yuhong Liu, Yuan Wei, Jin Zhang, Jinzha Zhang, Qing Xia, Qiang Zhang, Wenbo Zhao, Xiaoqiang Chen,