| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 7144864 | Sensors and Actuators B: Chemical | 2016 | 7 Pages |
Abstract
- Kanamycin is an aminoglycoside antibiotic, broadly used in human and veterinary for treatment of Gram-negative and Gram-positive infectious diseases.
- Overdosage of kanamycin could cause the serious side effects on human such as nephrotoxicity and ototoxicity.
- In this study, we developed a novel selective and sensitive fluorescent aptasensor for detection of kanamycin based on Exonuclease III and Gold nanoparticles with minimum consumption of aptamer for the first time.
- The designed aptasensor showed high selectivity toward aminoglycoside antibiotics, including kanamycin and gentamicin, with a limit of detection (LOD) as low as 437Â pM for kanamycin.
- The designed aptasensor was successfully used to detect kanamycin in serum and milk.
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Mohammad Ramezani, Noor Mohammad Danesh, Parirokh Lavaee, Khalil Abnous, Seyed Mohammad Taghdisi,