| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 7145044 | Sensors and Actuators B: Chemical | 2015 | 6 Pages |
Abstract
A dual carcinoembryonic antigen (CEA) biosensing alternative optoelectric platforms has been developed based on gold modified ITO electrode. The new platform is constructed by assembly of a thiol-derivative-nanogold (TDN) labeled anti-CEA (TDN-anti-CEA) antibody on a gold/indium tin oxide (ITO) electrode to create a high CEA sensitive surface. The gold film deposited on ITO glass provides a desirable substrate for the immobilization of the TDN-anti-CEA antibody and improves the compatibility between nanogold and electrode which results in a significant amplification. By using the same TDN-anti-CEA antibody probe, the CEA (analyte) was analyzed using two different methods; electrochemical impedance spectroscopy (EIS) and ultraviolet (UV) spectroscopy, the results showed that the limit of detection was 1 and 2Â pg/ml by EIS and UV methods, respectively. Given their promising advantages, it is likely that dual platforms will evolve to be an attractive tool for use in many different fields.
Keywords
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Hongjuan Zeng, Dorothy Araba Yakoba Agyapong, Caixia Li, Ranlin Zhao, Hong Yang, Chunhui Wu, Yadong Jiang, Yiyao Liu,