| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 7145158 | Sensors and Actuators B: Chemical | 2016 | 25 Pages |
Abstract
Based on the selective reduction of nitro group to hydroxylamine or amino group by nitroreductase in the presence of NADPH as an electron donor, a near-infrared ratiometric fluorescent probe CyNNO2 was designed and synthesized. CyNNO2 containing a p-nitrobenzyl moiety as a reactive group can selectively respond to nitroreductase with a ratiometric fluorescence signal output. The limit of detection for nitroreductase is 0.0058Â ng/mL. Moreover, the probe can be used to image the endogenous nitroreductase in A549 cells under hypoxic condition.
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Authors
Dongjian Zhu, Lin Xue, Guoping Li, Hua Jiang,