Cholesterol biosensor based on inkjet-printed Prussian blue nanoparticle-modified screen-printed electrodes

Here we describe the construction and optimization of a cholesterol biosensor based on screen-printed electrodes (SPEs) modified with inkjet-printed Prussian blue nanoparticles (PBNPs). The deposition of PBNPs using inkjet printing led to the highly facile fabrication of sensors with excellent sensitivity and reproducibility for the measurement of H2O2. Further integration of the sensor with a microfabricated low volume (4 μL) sample cell allowed the measurement of cholesterol in serum with the addition of cholesterol oxidase. The biosensor exhibited a sensitivity to cholesterol of 2.1 μA/mM cm2 (r2 = 0.97, n = 5) and was linear in the range of 0-15 mM.