Simple one-pot preparation of chitosan-reduced graphene oxide-Au nanoparticles hybrids for glucose sensing

A facile and effective approach to prepare glucose biosensor was developed based on chitosan-reduced graphene oxide-Au nanoparticles (CS-RGO-AuNPs) hybrids modified Pt electrode. Here CS which has many amino groups along its macromolecular chains and possesses good hydrophilic capability was used as reductant and stabilizer to transform GO into RGO. And one-pot warm bath method was employed to fabricate CS-RGO-AuNPs hybrids. The nanocomposites provided a good micro-bioenvironment for glucose oxidase (GOD) to facilitate electrocatalysis of glucose. The resulting biosensor exhibited a wide linear range of 15 μM to 2.13 mM, with a remarkable sensitivity of 102.4 μA mM−1 cm−2, a detection limit of 1.7 μm estimated at a signal-to-noise ratio of 3 and fast response time (within 5 s). Moreover, it showed good reproducibility, anti-interference ability and long-term stability. It showed that as-prepared CS-RGO-AuNPs nanohybrids not only exhibit efficiency for enzyme immobilization but also are of great importance for amplifying the sensor response. So the current work could provide a feasible approach and potential platform to fabricate a variety of enzymatic amperometric sensors.