Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
7145533 | Sensors and Actuators B: Chemical | 2015 | 8 Pages |
Abstract
Here, we describe the use of a biolayer interferometry biosensor for the fast and sensitive detection of virus-specific antibodies from human serum samples. Norovirus-like particles and norovirus P-particles were used to functionalise the biosensor tip. The detection of antibodies directly from serum samples was challenging, but the addition of a metal chelator (DAB) combined with an anti-human horseradish peroxidase-tagged antibody enabled enhanced detection of virus-specific antibodies in serum dilutions up to 1:100,000. Biolayer interferometry provides results faster than an ELISA, with results in as little as 10-20Â min when using pre-functionalised sensors. Therefore, biolayer interferometry combined with DAB enhancement offers an attractive method for quick and sensitive quantification of biomolecules from complicated sample matrices.
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Authors
Sanna Auer, Tiia Koho, Hanni Uusi-Kerttula, Timo Vesikari, Vesna Blazevic, Vesa P. Hytönen,