A novel glucose sensor based on immobilization of glucose oxidase on the chitosan-coated Fe3O4 nanoparticles and the luminol-H2O2-gold nanoparticle chemiluminescence detection system

- The new glucose biosensor fabricated by covalent immobilization of GOX on MNPs-CS.
- Signal detection was performed by luminol-H2O2 CL system.
- The gold nanoparticle catalysis the enzymatic and the CL reactions.
- The optimal CL conditions were obtained using Box-Behnken designs.
- The LOD (3σ) and LDR were 4.3 × 10−7 M and 8.5 × 10−7-1.0 × 10−4 M, respectively.