Electrocatalytic oxidation and flow detection analysis of semicarbazide at based IrOx chemically modified electrodes

An IrOx modified electrode was characterized and proposed as an amperometric sensor for the detection of semicarbazide using cyclic voltammetry, chronoamperometry and amperometry techniques. The modified electrode was characterized as sensing probe in both acid and alkaline medium toward the electrocatalytic oxidation and detection of semicarbazide. A liquid chromatography approach based on the strong cation exchange mode (LC-SCX column) was used in acid conditions for the separation and analysis of semicarbazide in a synthetic mixture. Operating under a constant applied potential of 1.1 V vs. Ag/AgCl and using HClO4 as carrier electrolyte, the limit of detection (LOD) is attested at level of 0.1 μM (i.e., 7.5 μg L−1), while an excellent precision and temporal stability of the amperometric signal, was observed. The linear ranges spanning over three order of magnitude of the detection limit with a correlation coefficients higher than 0.995.