A microfabricated quantum dot-linked immuno-diagnostic assay (μQLIDA) with an electrohydrodynamic mixing element

We present a microfabricated quantum dot-linked immuno-diagnostic assay (μQLIDA) biosensor with an embedded mixing element. We adapted our previous capillary-based system into a microfabricated planar microchannel, which has been integrated with an active, electrothermal mixing component. With the active mixing element, the detection time was significantly reduced from about 3.5 h to 30 min in a case study of detecting myeloperoxidase (MPO). MPO is an inflammatory biomarker that is over-expressed in inflammatory conditions including inflammatory bowel disease and cardiovascular disease. The μQLIDA biosensor presented here is a low-cost, rapid immunofluorescence sensor that can detect nanomolar concentrations of MPO, with a working volume of 2 μl.