Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
7146389 | Sensors and Actuators B: Chemical | 2014 | 10 Pages |
Abstract
A biosensor based on glucose oxidase (GOx) immobilized on gold nanoparticles (Au-NPs) electrochemically predeposited on the surface of graphite rod (GR) electrode was developed (GOx/Au-NPs/GR). Main analytical characteristics of this biosensor were determined and compared with those determined using a biosensor setup without Au-NP modification (GOx/GR). The highest analytical signal of GOx/Au-NPs/GR electrode was observed after 13 nm Au-NP deposition on the electrode from 0.8 nmol Lâ1 solution lasting 20 min, when cyclic voltammetry was performed in the range from 0.0 to +1.0 V vs Ag/AgCl. The best analytical characteristics of the developed biosensor were obtained after 25 mg mLâ1 GOx immobilization on the Au-NPs/GR electrode. Analytical signal registered using GOx/Au-NPs/GR electrode was 2.08 times higher in comparison to GOx/GR electrode. The registered currents of both electrodes were linearly dependent on glucose concentration in the range of 0.1-10 mmol Lâ1. The developed GOx/Au-NPs/GR electrode was characterized by high sensitivity, which was equal to 101.02 μA mMâ1 cmâ2 in the linear glucose detection range. The limit of detection was 0.083 mmol Lâ1 with relative standard deviation of 6% for GOx/Au-NPs/GR electrode. This study demonstrates a successful practical exploitation of the developed biosensor in a human serum sample.
Keywords
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Natalija German, Arunas Ramanavicius, Almira Ramanaviciene,