Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
7146853 | Sensors and Actuators B: Chemical | 2014 | 23 Pages |
Abstract
To assess the two dimensional distribution of the extracellular potassium ion concentration ([K+]out) surrounding hippocampal slices, we developed a 128Â ÃÂ 128Â pixel type potassium ion-sensitive image sensor (PIS) composed of a charge-coupled-type pH image sensor and a poly(vinyl chloride) (PVC) plasticized membrane containing valinomycin, a potassium ion-sensitive ionophore. The potential slope was 50Â mV/decade for a potassium ion concentration between 10â3 and 10â1Â M. Although variations in the signal were not recorded in the absence of a brain slice or in areas outside of the slice position, significant changes in [K+]out were detected underneath the hippocampal slices. We successfully detected the glutamate-induced changes in [K+]out associated with gating through potassium ion channels in hippocampal neurons without a labeling process. [K+]out increased from 10Â mM to 50Â mM in the area between the sensor and slice stimulated with glutamate.
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Authors
Akiteru Kono, Takashi Sakurai, Toshiaki Hattori, Koichi Okumura, Makoto Ishida, Kazuaki Sawada,