Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
7147041 | Sensors and Actuators B: Chemical | 2014 | 9 Pages |
Abstract
A mediator-free electrochemical biosensor with CeO2-PANI nano-interface for sensing histamine using diamine oxidase (DAO) has been developed. CeO2-PANI core-shell nanoparticles were prepared by hydrothermal method. The field emission scanning electron microscopy (FE-SEM) revealed the aggregated spherical morphology of CeO2. The core-shell formation of CeO2-PANI was confirmed with field emission transmission microscopy (FE-TEM). The polycrystallinity of CeO2 and CeO2-PANI was confirmed using X-ray diffraction (XRD). Immobilization of DAO with CeO2-PANI was confirmed with Fourier transform infrared spectroscopy (FT-IR). Electrochemical studies were carried out through cyclic voltammetry and amperometry using modified GCE/CeO2-PANI/DAO as a working electrode, Ag/AgCl saturated with 0.1 M KCl as a reference electrode and platinum (Pt) wire as a counter electrode. The linear range was observed from 0.45 to 1.05 mM with a sensitivity of 724.94 μA cmâ2 mMâ1. Michaelis-Menten constant was calculated as 0.798 mM. It exhibited limit of detection of 48.7 μM, limit of quantification of 132.4 μM with a response time of <1 s and good shelf life of 86% till 18 days. Also the developed biosensor was applied on tiger prawn to estimate the histamine content.
Keywords
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Manju Bhargavi Gumpu, Noel Nesakumar, Swaminathan Sethuraman, Uma Maheswari Krishnan, John Bosco Balaguru Rayappan,