| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 7147154 | Sensors and Actuators B: Chemical | 2014 | 6 Pages |
Abstract
We describe a microfluidic device designed specifically for the reversible immobilisation of Schizosaccharomyces pombe (Fission Yeast) cells to facilitate live cell super-resolution microscopy. Photo-Activation Localisation Microscopy (PALM) is used to create detailed super-resolution images within living cells with a modal accuracy of >25Â nm in the lateral dimensions. The novel flow design captures and holds cells in a well-defined array with minimal effect on the normal growth kinetics. Cells are held over several hours and can continue to grow and divide within the device during fluorescence imaging.
Keywords
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Laurence Bell, Ashwin Seshia, David Lando, Ernest Laue, Matthieu Palayret, Steven F. Lee, David Klenerman,