| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 7147852 | Sensors and Actuators B: Chemical | 2014 | 5 Pages |
Abstract
A simple and sensitive DNAzyme-based colorimetric sensor for detecting Hg2+ based on T-Hg2+-T base pair and magnetic beads (MBs) is proposed. In the presence of Hg2+, capture probes (CPs) immobilized on MBs are hybridized with the signal probes (SPs) consisting of G-quadruplex sequences with the help of T-Hg2+-T base pairs. The SPs are separated by MBs and used to bind hemin for the formation of catalytically active hemin/G-quadruplex DNAzymes, which catalyzes the H2O2-mediated oxidation of ABTS to produce a blue-green-colored product. A linear relationship between the absorbance and Hg2+ concentration is observed over a range of 10-200Â nM with a limit of detection 5Â nM. Taking advantage of MBs separation/collection process, the sensing system can be regenerated by using EDTA as the chelator.
Keywords
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Xijing Huang, Yanlin Hao, Hanyin Wu, Qingquan Guo, Liangqia Guo, Jing Wang, Liangshuang Zhong, Tianran Lin, FengFu Fu, Guonan Chen,