Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
7148158 | Sensors and Actuators B: Chemical | 2013 | 8 Pages |
Abstract
A label-free fiber-optic Fabry-Perot interferometric (FPI) immunosensor is proposed by layer-by-layer (LBL) self-assembly of a suspended chitosan and polystyrene sulfonate membrane at the tip of a hollow fiber. Immunoglobulin G (IgG) was immobilized on the polyelectrolyte substrate and the detection of target anti-immunoglobulin G (anti-IgG) was monitored through the measurement of substrate's effective optical thickness. The sensitivity and specificity of the sensor were studied using correlated and non-correlated anti-IgG/IgG pair. Sensitivity of 0.033 μm/(pg/mm2) and limit of detection of 0.005 nM was achieved with the sensor displaying minimal response toward non-correlated anti-IgG. The results demonstrate the feasibility of the sensor for immunosensing.
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Authors
L.H. Chen, C.C. Chan, R. Menon, P. Balamurali, W.C. Wong, X.M. Ang, P.B. Hu, M. Shaillender, B. Neu, P. Zu, Z.Q. Tou, C.L. Poh, K.C. Leong,