Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
7148279 | Sensors and Actuators B: Chemical | 2013 | 8 Pages |
Abstract
A method for the simultaneous determination of hydrochlorothiazide (HCTZ) and losartan (LOS) in pharmaceutical formulations using differential-pulse voltammetry (DPV) was developed. Two very well-resolved and reproducible oxidation peaks of HCTZ and LOS, with separation of 0.23 V, were obtained in Britton-Robinson (BR) buffer (pH 9.5) using an anodically pretreated boron-doped diamond electrode. Under the optimum analytical experimental conditions, the voltammetric method exhibited linear responses for simultaneous determination of HCTZ and LOS in the concentration range 3.0 Ã 10â6 to 7.4 Ã 10â5 mol Lâ1 for both compounds, with detection limits of 1.2 Ã 10â6 mol Lâ1 and 9.5 Ã 10â7 mol Lâ1, respectively. The proposed method was successfully applied in the simultaneous determination of LOS and HCTZ content in pharmaceutical formulations, whose accuracy was attested by good agreement of the results (paired t-test at a 95% confidence level) with those obtained using high performance liquid chromatography (HPLC).
Keywords
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Michely Cristina Gardenal Santos, César Ricardo Teixeira Tarley, Luiz Henrique Dall'Antonia, Elen Romão Sartori,