Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
7148807 | Sensors and Actuators B: Chemical | 2013 | 27 Pages |
Abstract
We report results of studies relating to the development of an electrochemical immunosensor based on carboxylated multiwalled carbon nanotubes (c-MWCNTs) electrophoretically deposited onto indium tin oxide (ITO) glass. This c-MWCNTs/ITO electrode surface has been functionalized with monoclonal aflatoxin B1 antibodies (anti-AFB1) for the detection of aflatoxin-B1 using electrochemical technique. Electron microscopy, X-ray diffraction and Raman studies suggest successful synthesis of c-MWCNTs and the Fourier transform infra-red spectroscopic (FT-IR) studies reveal its carboxylic functionalized nature. The proposed immunosensor shows high sensitivity (95.2 μA ngâ1 mL cmâ2), improved detection limit (0.08 ng mLâ1) in the linear detection range of 0.25-1.375 ng mLâ1. The low value of association constant (0.0915 ng mLâ1) indicates high affinity of immunoelectrode towards aflatoxin (AFB1).
Keywords
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Chandan Singh, Saurabh Srivastava, Md. Azahar Ali, Tejendra K. Gupta, Gajjala Sumana, Anchal Srivastava, R.B. Mathur, Bansi D. Malhotra,