Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
7148956 | Sensors and Actuators B: Chemical | 2013 | 6 Pages |
Abstract
The new composite films composed of diblock weak polyelectrolyte poly(2-hydroxyethyl methacrylate)-b-poly(2-(dimethylamino)ethyl methacrylate) (PHEMA-b-PDMAEMA, noted as PHD in the later content) and multi-walled carbon nanotubes (MWCNTs) were applied to immobilize hemoglobin (Hb) for biosensor fabrication. The characterization of Hb/PHD/MWCNTs films were demonstrated by ultraviolet-visible (UV-vis) spectra, scanning electron microscopy (SEM), electrochemical impedance spectroscopy (EIS), cyclic voltammetry (CV) and typical amperometric response (i-t) measurements. The immobilized Hb maintains its bioactivities and displays an excellent electrochemical behavior. The modified electrode exhibited good electrocatalytic activity to the reduction of hydrogen peroxide (H2O2). The linear response range of the H2O2 biosensor was from 1.0Â ÃÂ 10â6 to 1.5Â ÃÂ 10â3Â M with a detection limit of 3.5Â ÃÂ 10â7Â M. The apparent Michaelis-Menten constant of Hb on the PHD/MWCNTs film was estimated to be 0.51Â mM. These results indicated that the composite films have potential applicability of new types third-generation biosensors or bioreactors based on direct electrochemistry of the proteins.
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Authors
Zhou Wang, Jie Yi, Sui Yang,