Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
7148980 | Sensors and Actuators B: Chemical | 2013 | 8 Pages |
Abstract
A novel amperometric glucose biosensor is fabricated with immobilization of glucose oxidase (GOx) onto PAMAM-Fc dendrimers decorated gold electrode. Series of asymmetric PAMAM dendrimers containing a single ferrocene unit located in the focal point of these macromolecules have been synthesized and characterized. Surface of the gold electrode was covalently modified with 3-mercaptopropionic acid, PAMAM-Fc dendrimers and GOx enzyme by using coupling agents, respectively. The PAMAM-Fc/GOx biosensor shows an excellent performance for glucose at +0.25 V with a high sensitivity (6.54 μA/mM) and lower response time (â¼3 s) in a wide concentration range of 1-22 mM (correlation coefficient of 0.9988). In addition, PAMAM-Fc/GOx biosensor possesses better reproducibility, storage stability and there is negligible interference from other electroactive components.
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Mehmet Åenel, Cevdet Nergiz, Emre Ãevik,