Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
7149211 | Sensors and Actuators B: Chemical | 2013 | 6 Pages |
Abstract
A facile amperometric uric acid sensor was constructed by immobilizing uricase on gold/amino acid nanocomposites. The resultant biosensor had a short response time of less than 4 s, a sensitivity of 108 μA mMâ1 cmâ2, and a wide linear range from 0.02 to 2.5 mM. Its experimental detection limit is 7.0 μM (S/N = 3) and the apparent Michaelis-Menten constant is calculated to be 1.78 mM. Furthermore, it has been revealed that the biosensor exhibits excellent thermal stability, good anti-interference ability and favorable stability over relatively long-term storage. The results show that gold/amino acid nanocomposites can provide a promising material for biosensor design and other biological applications.
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Authors
Yan Liu, Min Yuan, Lili Liu, Rong Guo,