A facile electrochemical uricase biosensor designed from gold/amino acid nanocomposites

A facile amperometric uric acid sensor was constructed by immobilizing uricase on gold/amino acid nanocomposites. The resultant biosensor had a short response time of less than 4 s, a sensitivity of 108 μA mM−1 cm−2, and a wide linear range from 0.02 to 2.5 mM. Its experimental detection limit is 7.0 μM (S/N = 3) and the apparent Michaelis-Menten constant is calculated to be 1.78 mM. Furthermore, it has been revealed that the biosensor exhibits excellent thermal stability, good anti-interference ability and favorable stability over relatively long-term storage. The results show that gold/amino acid nanocomposites can provide a promising material for biosensor design and other biological applications.