Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
7149899 | Sensors and Actuators B: Chemical | 2012 | 6 Pages |
Abstract
Reliable surface chemistry is necessary for immobilization of biological recognition molecules on sensor surfaces. A dry and rapid method of pulsed-plasma assisted grafting of amine surface functional groups, using ammonia gas on polyurethane surfaces is successfully demonstrated by using pulse cycle times of 1-180 s (on-off). One cycle of plasma treatment was found to generate higher amine groups as they tend to degrade upon longer exposure to the high-energy radicals. Successful amination of the polyurethane surfaces was demonstrated by Fourier transform infrared spectroscopy (FTIR). To establish the availability of the surface amine groups for reaction, 1 μm carboxylated polybeads were reacted to polyurethane-coated piezoelectric excited millimeter-sized cantilever (PEMC) sensors before and after plasma treatment. Scanning electron microscope micrographs of PEMC sensors exposed to the same concentration of beads show more than four-fold increase in the presence of beads post pulsed-plasma treatment. Application of this method for protein immobilization was established by BSA chemisorption on plasma treated PEMC sensors.
Keywords
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Harsh Sharma, Raj Mutharasan,