Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
7234879 | Biotechnology Reports | 2018 | 31 Pages |
Abstract
Echinocandin B is a potent antifungal against the majority of fungal pathogens and its biosynthesis occurred by ecd and hty gene clusters in Emericella rugulosa NRRL 11440. We elucidated the functional necessity of in-clustered transcription factor; ecdB in the production of echinocandin B. We deleted the ecdB gene and found that ÎecdB mutant has no significant effect on echinocandin B production. The expression level of most of the ecd and hty cluster genes was not significantly altered except few of them up-regulated in knockout strain. The complete abrogation in ecdB gene expression was observed in ÎecdB strain. However, the interactions of purified EcdB protein with DNA sequence of ecdA, ecdH, ecdK and ecdI promoter was confirmed in-vitro. Our results conclude that EcdB protein in-vitro binds to the ecdA, ecdH, ecdK and ecdI promoter but in-vivo, it could not significantly affect the gene expression and echinocandin B production in Emericella rugulosa.
Keywords
Echinocandin BGMMeCBCTABORFRT-PCRArginineArgcetyl trimethylammonium bromideICUintensive care unitmolecular evolutionary genetics analysisSPRbase pairkilo base pairMaximum parsimonyDAYHourTranscription factoropen reading frameUTR یا untranslated regions Un-translated regionMEGAwild typepolymerase chain reactionreverse transcription polymerase chain reactionPCRFunctional redundancyhigh performance liquid chromatographyHPLC
Related Topics
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Renewable Energy, Sustainability and the Environment
Authors
Arvind Kumar, Varun Jaiswal, Vinay Kumar, Amitava Dey, Antresh Kumar,