Fabrication of aminotriazole grafted gold nanoparticles films on glassy carbon electrode and its application towards the simultaneous determination of theophylline and uric acid

This paper describes the potentiodynamic formation of AuNPs film on glassy carbon (GC) and indium tin oxide (ITO) electrodes using aminotriazole (AT) functionalized AuNPs (AT-AuNPs) and the utilization of the resulting AT-AuNPs film for the simultaneous determination of adenosine inhibitor drug, theophylline (TP) and uric acid (UA). The potentiodynamically prepared AT-AuNPs film modified electrodes were characterized by cyclic voltammetry (CV), atomic force microscopy (AFM), X-ray photoelectron spectroscopy (XPS) and UV–vis spectroscopy. The prepared AT-AuNPs film modified electrode showed excellent electrocatalytic activity towards TP and UA. Therefore, it was successfully used for the simultaneous determination of them in a mixture. Further, the AT-AuNPs film modified electrode was effectively used for the selective determination of TP in the presence of 25-fold excess UA. Using amperometry, detection of 40 nM TP was obtained. The current responses of TP was increased linearly while increasing their concentrations from 4.0 × 10−8 M to 1.0 × 10−4 M and the detection limit was found to be 8.5 × 10−9 M (S/N = 3). The practical application of the present modified electrode was demonstrated by simultaneously determining the concentrations of TP and UA in human blood serum and urine samples.

Graphical abstractAminotriazole grafted gold nanoparticles were potentiodynamically fabricated on GC electrode and then used for the determination of adenosine inhibitor drug in the presence of uric acid.Figure optionsDownload full-size imageDownload as PowerPoint slide