Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
740462 | Sensors and Actuators B: Chemical | 2013 | 4 Pages |
Abstract
The proof of concept of a DNA-biosensor based on a two-electrode electrochemical setup and using a microelectrode as working electrode, well adapted for detection in microliter samples and miniaturization, is presented herein. A 23-base DNA-probe self-assembled monolayer was first formed onto a 50 μm-diameter gold surface. The microelectrode extremity was then immersed in a 50 μL DNA-target solution drop itself deposited onto a 2 mm-diameter gold counter electrode. Transduction occurs via long-range electron transfer, which is enhanced subsequently to hybridization, due to DNA-base π-stacking. Single mismatch detection of this first prototype was matched at room temperature in the nanomolar range without any optimization.
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
M. Lazerges, V.T. Tal, P. Bigey, D. Scherman, F. Bedioui,