Article ID Journal Published Year Pages File Type
740462 Sensors and Actuators B: Chemical 2013 4 Pages PDF
Abstract

The proof of concept of a DNA-biosensor based on a two-electrode electrochemical setup and using a microelectrode as working electrode, well adapted for detection in microliter samples and miniaturization, is presented herein. A 23-base DNA-probe self-assembled monolayer was first formed onto a 50 μm-diameter gold surface. The microelectrode extremity was then immersed in a 50 μL DNA-target solution drop itself deposited onto a 2 mm-diameter gold counter electrode. Transduction occurs via long-range electron transfer, which is enhanced subsequently to hybridization, due to DNA-base π-stacking. Single mismatch detection of this first prototype was matched at room temperature in the nanomolar range without any optimization.

Related Topics
Physical Sciences and Engineering Chemistry Analytical Chemistry
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