Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
741373 | Sensors and Actuators B: Chemical | 2016 | 6 Pages |
•A chronopotentiometric assay for microcystin-LR was reported.•A solid-contact ion-selective electrode was designed for potentiometric sensing.•The potentiometric assay allows efficient MC-LR detection based on enzyme-inhibition.
We report here on the development of a chronopotentiometric assay for microcystin-LR based on enzymatic inhibition. The inhibition of protein phosphatase by microcystin-LR can be sensed potentiometrically by using 4-nitrophenyl phosphate as an enzyme substrate. A solid-contact ion-selective electrode (ISE) with poly(3,4-ethylenedioxythiophene)-poly(styrenesulfonate) as a transduction layer has been designed for potentiometric biosensing using the pulsed galvanastatic technique. By applying an anodic current, the enzymatic generated p-nitrophenol can be extracted into the polymeric membrane with tetradodecylammonium tetrakis(4-chlorophenyl)- borate to produce the chronopotentiometric signal. Meanwhile, a controlled voltage was applied to refresh the membrane for multiple consecutive measurements. The proposed potentiometric assay showed a linear response for microcystin-LR in the range 1–100 μg/L with a detection limit of 0.5 μg/L (3σ). We believe that the proposed method can be employed for sensitive, rapid and reliable determination of analytes involved in enzyme inhibition.
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