| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 741609 | Sensors and Actuators B: Chemical | 2015 | 7 Pages |
•A biocompatible nanocomposite film of Au nanoparticles in silica spheres is prepared.•It is used for simultaneous determination of ascorbic acid (AA) and uric acid (UA).•It exhibits efficient electrocatalytic oxidation of both AA and UA.•Determination of UA in human blood serum is successfully demonstrated.
This work reports the individual as well as simultaneous determination of ascorbic acid (AA) and uric acid (UA) by glassy carbon electrode (GCE) modified with a nanocomposite film of gold nanoparticles (Au NPs) inclusively adhered to MCM-41 type mesoporous silica spheres (Au-MSS). The impregnation of Au NPs into mesoporous silica spheres (MSS) is well established by surface characterization and electrochemical techniques. The Au-MSS modified GCE (GCE/Au-MSS) exhibits stable redox peaks, characteristics of Au NPs. Further, GCE/Au-MSS shows effective electrocatalytic oxidation of both AA and UA diminishing the overpotentials by 580 and 405 mV, respectively, than at bare GCE. Similarly, GCE/Au-MSS diminishes the oxidation overpotentials of AA and UA by 200 and 210 mV, respectively, relative to GCE/MSS. Differential pulse voltammetry technique is employed for the simultaneous determination of AA and UA in the presence of one another. The limit of detection is found to be 1.65 μM and 2.14 μM for AA and UA, respectively. The kinetic parameters like diffusion coefficient and the catalytic rate constant for the AA and UA electro-oxidations have been computed using chronoamperometric techniques. High sensitivity is displayed by the modified electrode and has been successfully employed for the analysis of UA in human blood serum.
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