| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 742011 | Sensors and Actuators B: Chemical | 2014 | 8 Pages |
•Development of a novel multipotentiostat system.•Capable of measuring simultaneous amperometric and potentiometric signals in up to six individual cells.•Low levels of crosstalk between instruments used in conjunction.•Software and modular design allows for flexibility for future experimental needs.
Multianalyte microphysiometry is a powerful technique for studying cellular metabolic flux in real time. Monitoring several analytes concurrently in a number of individual chambers, however, requires specific instrumentation that is not available commercially in a single, compact, benchtop form at an affordable cost. We developed a multipotentiostat system capable of performing simultaneous amperometric and potentiometric measurements in up to eight individual chambers. The modular design and custom LabVIEW™ control software provide flexibility and allow for expansion and modification to suit different experimental conditions. Superior accuracy is achieved when operating the instrument in a standalone configuration; however, measurements performed in conjunction with a previously developed multianalyte microphysiometer have shown low levels of crosstalk as well. Calibrations and experiments with primary and immortalized cell cultures demonstrate the performance of the instrument and its capabilities.
