| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 742112 | Sensors and Actuators B: Chemical | 2013 | 6 Pages |
Abstract
Polyethylene imine (PEI) based immobilization of antibodies is described and the concept is proved on the label free assay of C-Reactive Protein (CRP). This novel immobilization method is composed of a hyperbranched PEI layer which was deposited at a high pH (9.5) on the sensor surface. The free amino groups of PEI were derivatized with neutravidin by Biotin N-hydroxysuccinimide ester and the biotinylated anti-CRP antibody immobilized on this layer. Direct binding assay of recombinant CRP was successfully performed in the low μg/ml concentrations using a label free optical waveguide biosensor.
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
S. Kurunczi, A. Hainard, K. Juhasz, D. Patko, N. Orgovan, N. Turck, J.C. Sanchez, R. Horvath,