| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 742324 | Sensors and Actuators B: Chemical | 2013 | 8 Pages |
Abstract
Chemical oxidation of glycoprotein-bound thiophene-3-boronic acid (TBA) monomer by relatively mild oxidant chloroplatinate in neutral aqueous solution containing uricase yielded uricase–PTBA–Ptnano bionanocomposite with high enzymatic specific activity on a platinized (Ptplate) Au electrode, which was then covered by an outer-layer chitosan (CS) film to fabricate a CS/uricase–PTBA–Ptnano/Ptplate/Au electrode. This electrode exhibited a linear amperometric response to uric acid concentration from 5 μM to 1.2 mM with a sensitivity of 134 μA mM−1 cm−2, a limit of detection (S/N = 3) of 1 μM, and excellent operation/storage stability, which also worked well in serum samples.
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Yi Huang, Lijuan Bu, Wen Wang, Xiaoli Qin, Zou Li, Zhao Huang, Yingchun Fu, Xiaoli Su, Qingji Xie, Shouzhuo Yao,