Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
742440 | Sensors and Actuators B: Chemical | 2012 | 8 Pages |
Abstract
In this contribution we present a label-free continuous separation device for suspended-grown biological cells based on traveling wave dielectrophoresis (twDEP). Suspended-grown cells could otherwise only be separated by flow-cytometry which requires elaborate labeling steps. The chip consists of a structured PDMS layer on glass in which the channels have been defined. Parallel electrodes positioned along the microfluidic channel were used to expose selected cells to a twDEP force perpendicular to a pressure driven flow. With this device we have successfully separated viable Saccharomyces cerevisiae and Jurkat (T-cell leukemia) cells from a mix also containing cell culture debris, non-viable cells, and Lactobacillus casei bacteria.
Keywords
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Sander van den Driesche, Vivek Rao, Dietmar Puchberger-Enengl, Wojciech Witarski, Michael J. Vellekoop,