Article ID Journal Published Year Pages File Type
742497 Sensors and Actuators B: Chemical 2012 7 Pages PDF
Abstract

A simple and efficient molecularly imprinted polymers (MIPs) based electrochemical sensor were proposed and prepared by electropolymerization of pyrrole in the presence of bovine hemoglobin (BHb) in an aqueous solution. The fabrication process of the sensor was characterized by differential pulse voltammetry and electrochemical impedance spectroscopy, in which [Fe(CN)6]3−/[Fe(CN)6]4− was used as an electrochemical active probe. The influence factors including electropolymerization cycles, scan rate, the concentration of pyrrole, and the extraction conditions were investigated in detailed. Under the optimized conditions, the experimental results showed that the MIPs based sensor possessed a fast rebinding dynamics and an excellent recognition capacity to BHb, compared to the other non-template proteins. Moreover, the prepared sensor also exhibited a dependent relationship between the concentration of template protein and peak current of [Fe(CN)6]3−/[Fe(CN)6]4−.

Related Topics
Physical Sciences and Engineering Chemistry Analytical Chemistry
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