Article ID Journal Published Year Pages File Type
743354 Sensors and Actuators B: Chemical 2012 5 Pages PDF
Abstract

Variations in calcium ion (Ca2+) concentrations play a crucial role in cell–cell communication; periodic changes act as cellular signals. In this study, we developed a solid-state calcium ion-selective electrode (Ca2+ ISE) integrated in a microchannel to measure extracellular Ca2+ variations; an ISE has a high detection sensitivity (as low as 10−9 M) and selectivity against potassium ions (K+). The sensor was used to monitor the cellular activities of HepG2/C3As cultured in a microchannel. We measured a periodically oscillating extracellular Ca2+ concentration that was superimposed on a gradual increase in the Ca2+ concentration after potassium chloride (KCl) stimulation. The oscillation period was in the range of 30–50 s, which was in good agreement with the literature.

Related Topics
Physical Sciences and Engineering Chemistry Analytical Chemistry
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