Article ID Journal Published Year Pages File Type
743410 Sensors and Actuators B: Chemical 2009 4 Pages PDF
Abstract

A single-use, disposable biosensor prototype of fructosyl valine (FV) was developed in this study. FV was a model compound for the glycosylated hemoglobin (HbA1c) detection and the measurement of HbA1c level was important for the long-term management of diabetic patients. Therefore, the detection of FV would be important for diabetic patient management as a means for HbA1c biosensing, particularly if this biosensor could be a single-use, disposable and manufactured cost effectively.This single-use, disposable FV electrochemical biosensor was fabricated using thick film screen-printing technique. Iridium nano-particle catalysts were incorporated in the carbon-based working and counter electrodes enhancing the performance of the biosensor for the FV detection. The enzyme fructosyl amine oxidase (FAO, EC 1.5.3, 0.45 U mg−1 of solid from recombinant Escherichia coli) was immobilized on the working electrode of the biosensor prototype. This biosensor operated at a relatively low electrochemical potential (+0.25 V versus Ag/AgCl) at ambient temperature to detect the enzymatically produced H2O2 from FV. This biosensor responded well to the FV level in a physiological range and the sensor output could be modeled by the Michaelis–Menton relationship. Thus, this biosensor prototype could be used effectively as the basis for a single-use, disposable HbA1c detection for diabetic patient management.

Related Topics
Physical Sciences and Engineering Chemistry Analytical Chemistry
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