Impedance study of the hybrid molecule alginate–pyrrole: Demonstration as host matrix for the construction of a highly sensitive amperometric glucose biosensor

The aim of this paper is to report the characterization and application of a new and attractive hybrid molecule (alginate modified with pyrrole) alginate-pyrrole (Alg–py) with glucose oxidase (GOx) to create a highly responsive glucose biosensor. The biosensor was built by covalent attachment of GOx with the Alg-py matrix, followed by pyrrole polymerization, then casting the biocomposite onto the surface of a platinum electrode (Pt). The unique three-dimensional structure, porous morphology, conductive setup, mechanical firmness and biocompatibility resulted in low response time (4 s), high sensitivity value (16 μA mM−1 cm−2) and a very low enzyme load (9.2 U).