Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
743564 | Sensors and Actuators B: Chemical | 2008 | 4 Pages |
Abstract
The hybridisation of thiol-modified single-stranded DNA (SH-ssDNA) embedded in lipoate or hydrophilic polymer monolayers on gold have been studied through the technique of surface plasmon resonance (SPR). SH-ssDNA and the blocking agents were co-adsorbed on gold from the same solution or the surface was post-treated with the blocking agents. The assembling process is allowed to take place for only 10 min. Binding of non-complementary and complementary DNA is dependent both on the blocking agent and on the assembling method used. The lowest non-complementary binding and the highest complementary binding of DNA was obtained with a layer assembled from a binary solution of SH-ssDNA and the lipoate blocking agent.
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Inger Vikholm-Lundin, Reetta Piskonen,