Fabrication of a novel electrochemiluminescence glucose biosensor using Au nanoparticles decorated multiwalled carbon nanotubes

Au nanoparticles (nanoAu) with an average diameter of 60 nm were decorated on the surface of multiwalled carbon nanotubes to prepare MWCNTs–nanoAu nano-hybrids. The MWCNTs–nanoAu nano-hybrids were cast on the surface of a glassy carbon electrode and were then further modified with a layer comprising glucose oxidase and chitosan to fabricate a novel electrochemiluminescence (ECL) glucose biosensor. The biosensor showed a remarkably improved electrocatalytic activity towards luminol oxidation and significant improvement in its ECL response. The proposed ECL biosensor exhibited excellent performance for glucose detection with a wide linear range (1–1000 μM), low detection limit (0.5 μM), excellent reproducibility (0.5%) and satisfactory selectivity.