Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
744776 | Sensors and Actuators B: Chemical | 2010 | 4 Pages |
Abstract
Compact discs (CDs) can be used as rapid, low-cost, high-capacity screening platforms for running bioassays with no modification of the detection hardware (conventional standard optical drive). We describe herein a new protocol to read and quantitate biotin–streptavidin binding assays with a standard optical drive by using a CD-data analysis software (IsoBuster), which identifies erroneous sectors by locating the exact error position bit-by-bit and allows various data formats to be used. The numbers of erroneous sectors increase as a function of the streptavidin concentration in the tested solutions. High spatial accuracy and detection sensitivity (0.3 μg/mL) were achieved.
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Manu Pallapa, Lily M.L. Ou, M. (Ash) Parameswaran, Hua-Zhong Yu,