Article ID Journal Published Year Pages File Type
746299 Sensors and Actuators B: Chemical 2006 7 Pages PDF
Abstract

An amperometric immunosensor was developed based on immobilizing antibody on the surface of modified CdFe2O4 magnetic nanoparticles. The goat anti-human IgG antibody (anti-IgG) was first covalently immobilized to core–shell (CdFe2O4–SiO2) magnetic nanoparticles, which were surface-modified with amino groups on the surface. The resulting bio-magnetic nanoparticles were attached to the surface of carbon paste electrode (CPE) with the help of a permanent magnet. A sandwich immunoassay was performed on the bio-magnetic nanoparticles monolayer supported by CPE. The assay comprised first loading of IgG on the bio-magnetic nanoparticles monolayer, then blocking in BSA solution, followed by incubation in anti-IgG-HRP conjugate, and finally the amperometric detection with hydroquinone as a mediator. The linear range for IgG assay was 0.51–30.17 μg ml−1 and the detection limit was 0.18 μg ml−1. The simple manipulations of construction and low cost were main advantages of proposed immunosensing method.

Related Topics
Physical Sciences and Engineering Chemistry Analytical Chemistry
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