| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 746332 | Sensors and Actuators B: Chemical | 2009 | 5 Pages |
Abstract
A new versatile chemistry on gold surface has been developed and studied with a Surface Plasmon Resonance Imaging system. It is based on a monolayer of homobifunctional cross-linker 1,4 phenylenediisothiocyanate (PDC) modified with neutravidin bounded onto a gold film coated with a self-assembled monolayer of β-mercaptoethylamine (cysteamine). Using this surface chemistry, a DNA microarray affinity sensor has been developed. Specificity, regenerability and surface homogeneity were quantified and successfully applied to both synthetic oligonucleotides and PCR-amplified products DNA–DNA interactions.
Keywords
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Jolanda Spadavecchia, Julien Moreau, Jérôme Hottin, Michael Canva,