Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
747142 | Sensors and Actuators B: Chemical | 2006 | 4 Pages |
Abstract
The salivary glands of the blowfly were injected with luminescent oxygen-sensitive microbeads. The changes in oxygen content within individual gland tubules during hormone-induced secretory activity were quantified. The measurements are based on an upgraded phase-modulation technique, where the phase shift of the sensor phosphorescence is determined independently from concentration and background signals. We show that the combination of a lock-in amplifier with a fluorescence microscope results in a convenient setup to measure oxygen concentrations within living animal tissues at the cellular level.
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Elmar Schmälzlin, Bernd Walz, Ingo Klimant, Bettina Schewe, Hans-Gerd Löhmannsröben,