| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 750680 | Sensors and Actuators B: Chemical | 2015 | 8 Pages |
In this paper, we developed a new selectively fluorescent probe for Hg2+ based on BODIPY. The probe exhibited strong affinity for Hg2+ over other metal ions. Treatment with Hg2+ would “turn on” the fluorescence of the probe, and strong green fluorescence could be observed. The binding constant (Ka) of the probe with Hg2+ was calculated to be 9.43 × 104 M−1 from a Benesi–Hildebrand plot and the detection limit was evaluated to be as low as 1.73 nM (LOD = 3σ/slope, σ is standard deviation). Biological applications were investigated employing living HeLa cells, zebrafish and nude mouse.
Graphical abstractA fluorescent probe for imaging Hg2+ was prepared. The probe was successfully applied in the imaging of Hg2+ in living HeLa cells, zebrafish and living nude mouse.Figure optionsDownload full-size imageDownload as PowerPoint slide
