Electrochemical characterization of enzymatic organo-metallic coating of TiO2 nanoparticles

•Role of triglyceride molecules in natural binders to support binding of TiO2 nanoparticles.•Chemical functionality of TiO2 nanoparticles because of these binders.•Effectiveness of this functionality for enzyme immobilization/attachment.•Potential of these surfaces in electrochemical biosensors applications.

Triglyceride molecules in acrylated epoxidized soybean oil (AESO) and maleinized acrylated epoxidized soybean oil (MAESO) base binders were used to achieve binding of TiO2 nanoparticles on conducting glass surfaces. These surfaces were coated with glucose oxidase enzyme after annealing at 450 °C. The annealed film surface was examined by SEM and AFM observations confirm enhancement of surface porosity and morphology, before and after enzyme attachment. Electrochemical techniques were used to study the glucose sensing properties of these substrates prepared with these natural plant oils binders. It has been observed that substrates prepared by using AESO and MAESO fatty acid binders have higher enzyme adsorption capability and exhibit better charge transport properties.